Sphingolipid metabolism and cell cycle

We are studying the reciprocal regulation of cell cycle and sphingolipid metabolism

Sphingolipids are central bioactive lipids that have important functions in cell division.
However, how cells control sphingolipid metabolism during their division is not well
understood. To answer this question, we use S. cerevisiae as a model.

Studying lipid metabolism

yarrowiaYarrowia lipolytica  is a dimorphic fungus that grows in the form of yeast and as filaments. It has called the attention of scientists for its growth in harsh  conditions.  It can be genetically modified to produce more lipids as shown in the figure  where lipid droplets were stained with Bodipy and yeast were observed by fluorescence microscopy.  The main challenge is to create mutants  in new environmental isolates!

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LABORATORY MOLECULAR BIOLOGY OF YEAST

 

Our group is interested in the potential use of  yeast  as work-horse for lipid production for biotechnological applications. The focus of our most recent work is to study the signaling mechanisms that alter the accumulation of lipid droplets in yeast, using S. cerevisiae, Yarrowia lipolytica and Usitlago maydis. We use biochemistry, yeast genetics, microarray and systems biology approaches. We had previously reported a rapid high throughput method to study lipid droplet dynamics in the  known yeast  Saccharomyces cerevisiae  Bozaquel-Morais BL, PLoS One. 2010 Oct 28;5(10):e13692) that have enable us to discover in a simple manner genes that are involved in lipid metabolisM

 

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Monica Montero Lomeli

Associate Professor Universidade Federal do Rio de Janeiro,

Instituto de Bioqímica  Médica-Programa de Biologia Molecular e Biotecnologia

CLAUDIO AKIO MASUDA

Associate Professor Universidade Federal do Rio de Janeiro,

Instituto de Bioqímica  Médica-Programa de Biologia Molecular e Biotecnologia